HAND1 Mouse Monoclonal Antibody [Clone ID: OTI1G10]

CAT#: TA502671

HAND1 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HAND1 (Cat# RC200513, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1(Cat# TA502671). Positive lysates LY417727 (100ug) and LC417727 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Anti-HAND1 mouse monoclonal antibody (TA502671) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1 (RC200513).

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  Flow cytometric Analysis of Hela cells, using anti-HAND1 antibody (TA502671), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-HAND1 antibody (TA502671), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1G10
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HAND1 (NP_004812) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.4 kDa
• Gene Name: heart and neural crest derivatives expressed 1
• Database Link:
  NP_004812
  Entrez Gene 15110 MouseEntrez Gene 59112 RatEntrez Gene 9421 Human
  UniProt ID O96004
• Background: The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq]
• Synonyms: bHLHa27; eHand; Hxt; Thing1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors