RASSF1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI8E4]

CAT#: TA502487BM

RASSF1 mouse monoclonal antibody, clone OTI8E4 (formerly 8E4), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RASSF1 (Cat# RC213525, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1(Cat# TA502487). Positive lysates LY402099 (100ug) and LC402099 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RASSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502487)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RASSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502487)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RASSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502487)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RASSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502487)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RASSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502487)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RASSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502487)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RASSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502487)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RASSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502487)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RASSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502487)

• Anti-RASSF1 mouse monoclonal antibody (TA502487) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1 (RC213525).

• HEK293T cells transfected with either RC213525 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RASSF1 antibody (TA502487), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-RASSF1 antibody (TA502487), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI8E4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-341 of human RASSF1(NP_009113) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.6 kDa
• Gene Name: Ras association domain family member 1
• Database Link:
  NP_009113
  Entrez Gene 56289 MouseEntrez Gene 363140 RatEntrez Gene 11186 Human
  UniProt ID Q9NS23
• Background: This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
• Synonyms: 123F2; NORE2A; RASSF1A; RDA32; REH3P21

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Bladder cancer, Non-small cell lung cancer, Pathways in cancer