EIF4EL3 (EIF4E2) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1F2]

CAT#: TA502404BM

EIF4E2 mouse monoclonal antibody, clone OTI1F2 (formerly 1F2), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E2 (RC201391, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E2. Positive lysates LY401518 (100ug) and LC401518 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF4E2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EIF4E2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502404)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EIF4E2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502404)

• 

  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-EIF4E2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502404)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EIF4E2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502404)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EIF4E2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502404)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EIF4E2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502404)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EIF4E2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502404)

• Flow cytometric Analysis of Hela cells, using anti-EIF4E2 antibody (TA502404), (Red), compared to a nonspecific negative control antibody, (Blue).

Show all 10 images

Specifications

Product Data

• Clone Name: OTI1F2
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EIF4E2 (NP_004837) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.2 kDa
• Gene Name: eukaryotic translation initiation factor 4E family member 2
• Database Link:
  NP_004837
  Entrez Gene 26987 MouseEntrez Gene 9470 Human
  UniProt ID O60573
• Synonyms: 4E-LP; 4EHP; EIF4EL3; IF4e

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway