CD147 (BSG) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5D8]

CAT#: TA501190BM

Anti-BSG (CD147) mouse monoclonal antibody, clone OTI5D8 (formerly 5D8), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (RC219464, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG. Positive lysates LY419777 (100ug) and LC419777 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-BSG monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501190, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501190, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501190, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501190, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501190, Dilution 1:50)

• Anti-BSG mouse monoclonal antibody (TA501190) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG (RC219464).

• HEK293T cells transfected with either RC219464 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BSG antibody (TA501190), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-BSG antibody (TA501190), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-BSG antibody (TA501190), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI5D8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BSG(NP_001719) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.2 kDa
• Gene Name: basigin (Ok blood group)
• Database Link:
  NP_001719
  Entrez Gene 682 Human
  UniProt ID P35613
• Background: The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: 5F7; CD147; EMMPRIN; OK; TCSF

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane