DAP Kinase 2 (DAPK2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1C5]

CAT#: TA501099AM

DAPK2 (DAP Kinase 2 ) mouse monoclonal antibody, clone OTI1C5 (formerly 1C5), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DAPK2 (Cat# RC216274, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAPK2(Cat# TA501099).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-DAPK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501099)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501099)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501099)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501099)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501099)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501099)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501099)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DAPK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501099)

• Anti-DAPK2 mouse monoclonal antibody (TA501099) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DAPK2 (RC216274).

• Immunoprecipitation (IP) of DAPK2 by using TrueMab monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-DAPK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• HEK293T cells transfected with either RC216274 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAPK2 antibody (TA501099), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-DAPK2 antibody (TA501099), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1C5
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.7 kDa
• Gene Name: death associated protein kinase 2
• Database Link:
  NP_055141
  Entrez Gene 13143 MouseEntrez Gene 300799 RatEntrez Gene 610682 DogEntrez Gene 706421 MonkeyEntrez Gene 23604 Human
  UniProt ID Q9UIK4
• Background: This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq]
• Synonyms: DRP-1; DRP1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Pathways in cancer