NIT2 Mouse Monoclonal Antibody [Clone ID: OTI3D5]

CAT#: TA501097S

Anti-NIT2 mouse monoclonal antibody, clone OTI3D5 (formerly 3D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT2 (RC210660, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT2. Positive lysates LY402761 (100ug) and LC402761 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NIT2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and NIT2-Knockout 293T cells (KO, Cat# LC812235) were separated by SDS-PAGE and immunoblotted with anti-NIT2 monoclonal antibody TA501097, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501097, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501097, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (TA501098, Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)

• Anti-NIT2 mouse monoclonal antibody (TA501097) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2 (RC210660).

• HEK293T cells transfected with either pCMV6-ENTRY NIT2 (RC210660) (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT2 mouse monoclonal (TA501097), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3D5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.79 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.4 kDa
• Gene Name: nitrilase family member 2
• Database Link:
  NP_064587
  Entrez Gene 52633 MouseEntrez Gene 288174 RatEntrez Gene 56954 Human
  UniProt ID Q9NQR4
• Background: Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle
• Synonyms: HEL-S-8a