MCL1 Mouse Monoclonal Antibody [Clone ID: OTI2E11]

CAT#: TA500986

Anti-MCL1 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MCL1 (Cat# RC200521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1(Cat# TA500986). Positive lysates LY411855 (100ug) and LC411855 (20ug) can be purchased separately from OriGene.

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  Flow cytometric analysis of Jurkat cells, using anti-MCL1 antibody (TA500986), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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  Flow cytometric analysis of Hela cells, using anti-MCL1 antibody (TA500986), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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  Immunofluorescent staining of HepG2 cells using anti-MCL1 mouse monoclonal antibody (TA500986).

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  Anti-MCL1 mouse monoclonal antibody (TA500986) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1 (RC200521).

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  Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-MCL1 monoclonal antibody at 1:200 dilution.

• Flow cytometric analysis of HEK293T cells (black), stained with anti-MCL1 antibody (TA500986) (Blue) or Isotype IgG control antibody (Red).

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Specifications

Product Data

• Clone Name: OTI2E11
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, Flow 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.3 kDa
• Gene Name: MCL1 apoptosis regulator, BCL2 family member
• Database Link:
  NP_068779
  Entrez Gene 4170 Human
  UniProt ID Q07820
• Background: The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.
• Synonyms: bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM

Reference Data

• Protein Families: Druggable Genome, Transmembrane