Natriuretic Peptide Receptor C (NPR3) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2G2]

CAT#: TA500956AM

Anti-NPR3 (Natriuretic Peptide Receptor C) mouse monoclonal antibody, clone OTI2G2 (formerly 2G2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPR3 (RC219453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPR3. Positive lysates LY424462 (100ug) and LC424462 (20ug) can be purchased separately from OriGene.

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  Figure from citation: Western Blot of NPR3 protein level by using anti-NPR3 antibody in HEK293 cells. Dilution: 1:1000 View Citation

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NPR3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500956, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NPR3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500956, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NPR3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500956, Dilution 1:50)

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  Anti-NPR3 mouse monoclonal antibody (TA500956) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NPR3 (RC219453).

• HEK293T cells transfected with either pCMV6-ENTRY NPR3 (RC219453) (Red) or empty vector control plasmid (Blue) were immunostained with anti-NPR3 mouse monoclonal (TA500956), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2G2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NPR3 (NP_000899) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.8 kDa
• Gene Name: natriuretic peptide receptor 3
• Database Link:
  NP_000899
  Entrez Gene 18162 MouseEntrez Gene 25339 RatEntrez Gene 4883 Human
  UniProt ID P17342
• Background: The family of natriuretic peptides (see MIM 108780) elicit a number of vascular, renal, and endocrine effects that are important in the maintenance of blood pressure and extracellular fluid volume. These effects are mediated by specific binding of the peptides to cell surface receptors in the vasculature, kidney, adrenal, and brain
• Synonyms: ANP-C; ANPR-C; ANPRC; C5orf23; GUCY2B; NPR-C; NPRC

Reference Data

• Protein Families: Druggable Genome, Transmembrane