CISD1 Mouse Monoclonal Antibody [Clone ID: OTI2B3]

CAT#: TA500904

CISD1 mouse monoclonal antibody, clone OTI2B3 (formerly 2B3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CISD1 (Cat# RC203308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1(Cat# TA500904). Positive lysates LY413042 (100ug) and LC413042 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-CISD1 monoclonal antibody (1:200).

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  Anti-CISD1 mouse monoclonal antibody (TA500904) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1 (RC203308).

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  Immunofluorescent staining of HeLa cells using anti-CISD1 mouse monoclonal antibody (TA500904).

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  Flow cytometric Analysis of Jurkat cells, using anti-CISD1 antibody (TA500904), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI2B3
• Applications: FC, IF, WB
• Recommended Dilution: IHC 1:100~400, WB: 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CISD1 (NP_060934) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.72 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.0 kDa
• Gene Name: CDGSH iron sulfur domain 1
• Database Link:
  NP_060934
  Entrez Gene 52637 MouseEntrez Gene 294362 RatEntrez Gene 55847 Human
  UniProt ID Q9NZ45
• Background: CISD1 is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity (Wiley et al., 2007, 2007 [PubMed 17376863] [PubMed 17584744]). [supplied by OMIM]
• Synonyms: C10orf70; MDS029; mitoNEET; ZCD1

Reference Data

• Protein Families: Transmembrane