PKMYT1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2B4]

CAT#: TA500839BM

PKMYT1 mouse monoclonal antibody, clone OTI2B4 (formerly 2B4), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (RC222657, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1. Positive lysates LY401358 (100ug) and LC401358 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of Jurkat cells, using anti-PKMYT1 antibody (TA500839), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-PKMYT1 antibody (TA500839), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Anti-PKMYT1 mouse monoclonal antibody (TA500839) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKMYT1 (RC222657).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PKMYT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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Specifications

Product Data

• Clone Name: OTI2B4
• Applications: FC, IF, WB
• Recommended Dilution: IHC 1:100~200, WB: 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PKMYT1 (NP_004194) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.3 kDa
• Gene Name: protein kinase, membrane associated tyrosine/threonine 1
• Database Link:
  NP_004194
  Entrez Gene 268930 MouseEntrez Gene 287101 RatEntrez Gene 9088 Human
  UniProt ID Q99640
• Background: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
• Synonyms: MYT1; PPP1R126

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation