Non Neuronal Enolase (ENO1) Rabbit Monoclonal Antibody [Clone ID: R01-4C8]

CAT#: TA384174

ENO1 Rabbit monoclonal Antibody

Product Images

Western blot analysis of ENO1 in mouse brain, mouse heart lysates using ENO1 antibody.

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  Western blot analysis of ENO1 in Jurkat, rat Brain, C6, Hela lysates using ENO1 antibody.

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  Immunohistochemistry analysis of paraffin-embedded Human tonsil using ENO1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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  Immunocytochemistry analysis of ENO1(green) in Hela using ENO1 antibody,and DAPI(blue)

Specifications

Product Data

• Clone Name: R01-4C8
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/500
  ICC/IF: 1/100
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human ENO1
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 47 kDa; Observed MW: 47 kDa
• Gene Name: enolase 1
• Database Link:
  Entrez Gene 2023 Human
  UniProt ID P06733
• Background: Swiss-Prot Acc.P06733.Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.MiscellaneousUsed as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.
• Synonyms: ENO1L1; MBP-1; MBPB1; MPB-1; MPB1; NNE; PPH; tau-crystallin