CDK4 Rabbit Monoclonal Antibody [Clone ID: R04-8I5]
| Product Data | |
| Clone Name | R04-8I5 |
|---|---|
| Application | IF, IP, WB |
| Recommended Dilution | WB: 1/1000 ICC/IF: 1/50 IP: 1/20 |
| Reactivity | Human, Mouse |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Monoclonal |
| Immunogen | A synthetic peptide of human Cdk4 |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Concentration | lot specific |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Stability | 1 year |
| Shipping | Blue Ice |
| Predicted Protein Size | Calculated MW: 34 kDa; Observed MW: 34 kDa |
| Gene Name | cyclin-dependent kinase 4 |
| Database Link | |
| Background | Swiss-Prot Acc.P11802.Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| Synonyms | CMM3; MGC14458; PSK-J3 |
| Reference Data | |
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