TOMM20 Rabbit Polyclonal Antibody

CAT#: TA382698

TOM20 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using TOM20 antibody (TA382698) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Western blot analysis of extracts of various cell lines, using TOM20 antibody (TA382698) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunofluorescence analysis of C6 cells using [KO Validated] TOM20 Rabbit pAb (TA382698) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] TOM20 Rabbit pAb (TA382698) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using [KO Validated] TOM20 Rabbit pAb (TA382698) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of HeLa cells using TOM20 antibody (TA382698) at dilution of 1:100. Blue: DAPI for nuclear staining.

• Immunofluorescence analysis of L929 cells using TOM20 antibody (TA382698) at dilution of 1:100. Blue: DAPI for nuclear staining.

• Immunofluorescence analysis of U2OS cells using TOM20 antibody (TA382698) at dilution of 1:100. Blue: DAPI for nuclear staining.

• Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TOM20 antibody (TA382698). Western blot was performed from the immunoprecipitate using TOM20 antibody (TA382698) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:20 - 1:50
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 16kDa
• Gene Name: translocase of outer mitochondrial membrane 20
• Database Link:
  Entrez Gene 9804 Human
  UniProt ID Q15388
• Background: Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
• Synonyms: KIAA0016; MAS20; MGC117367; MOM19; TOM20