T Rabbit Polyclonal Antibody

CAT#: TA382262

Brachyury Rabbit polyclonal Antibody

Product Images

WesBrachyuryern bloBrachyury analysis of exBrachyuryracBrachyurys of H460 cells, using Brachyury Rabbit pAb (TA382262) aBrachyury 1:1000 diluBrachyuryion.|Secondary anBrachyuryibody: HRP GoaBrachyury AnBrachyuryi-RabbiBrachyury IgG (H+L) aBrachyury 1:10000 diluBrachyuryion.|LysaBrachyuryes/proBrachyuryeins: 25ug per lane.|Blocking buffer: 3% nonfaBrachyury dry milk in BrachyuryBSBrachyury.|DeBrachyuryecBrachyuryion: ECL Basic KiBrachyury .|Exposure Brachyuryime: 30s.

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  Immunofluorescence analysis of NCI-H460 cells using Brachyury Rabbit pAb (TA382262) aBrachyury diluBrachyuryion of 1:200 (40x lens). Blue: DAPI for nuclear sBrachyuryaining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IF,1:50 - 1:200
• Reactivities: Human, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: RecombinanBrachyury fusion proBrachyuryein conBrachyuryaining a sequence corresponding Brachyuryo amino acids 226-435 of human Brachyury (NP_003172.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 41kDa/47kDa
• Gene Name: T brachyury transcription factor
• Database Link:
  Entrez Gene 6862 Human
  UniProt ID O15178
• Background: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: MGC104817; OTTHUMP00000017588; TF; TFT