NSUN2 Rabbit Polyclonal Antibody

CAT#: TA379348

NSUN2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using NSUN2 antibody (TA379348) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunofluorescence analysis of C6 cells using NSUN2 antibody (TA379348) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using NSUN2 antibody (TA379348) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 508-767 of human NSUN2 (NP_060225.4).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 59kDa/82kDa/86kDa
• Gene Name: NOP2/Sun RNA methyltransferase family member 2
• Database Link:
  Entrez Gene 54888 Human
  UniProt ID Q08J23
• Background: This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
• Synonyms: FLJ20303; hTrm4; Misu; SAKI; TRM4