NF2 Rabbit Polyclonal Antibody

CAT#: TA379069

NF2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and NF2 knockout (KO) HeLa cells, using NF2 antibody (TA379069) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 6s.

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  Western blot analysis of extracts of various cell lines, using NF2 antibody (TA379069) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

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  Immunofluorescence analysis of C6 cells using NF2 antibody (TA379069) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using NF2 antibody (TA379069) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using NF2 antibody (TA379069) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug NF2 antibody (TA379069). Western blot was performed from the immunoprecipitate using NF2 antibody (TA379069) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2 (NP_000259.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 19-30kDa/59-72kDa
• Gene Name: neurofibromin 2 (merlin)
• Database Link:
  Entrez Gene 4771 Human
  UniProt ID P35240
• Background: This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts.
• Synonyms: ACN; BANF; merlin; Neurofibromin-2; SCH; schwannomerlin; Schwannomin