ILF3 Rabbit Polyclonal Antibody

CAT#: TA377489

ILF3 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ILF3 antibody (TA377489) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Western blot analysis of extracts of mouse thymus, using ILF3 antibody (TA377489) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 5s.

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  Immunofluorescence analysis of C6 cells using ILF3 antibody (TA377489) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH-3T3 cells using ILF3 antibody (TA377489) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using ILF3 antibody (TA377489) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug ILF3 antibody (TA377489). Western blot was performed from the immunoprecipitate using ILF3 antibody (TA377489) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ILF3 (NP_036350.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 74kDa/75kDa/76kDa/82kDa/95kDa
• Gene Name: interleukin enhancer binding factor 3
• Database Link:
  Entrez Gene 3609 Human
  UniProt ID Q12906
• Background: This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Synonyms: CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; NF-AT-90; NF90; NF110; NFAR; NFAR-1; NFAR2; TCP80; TCP110