CTCF Rabbit Polyclonal Antibody

CAT#: TA375185

CTCF Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of 293T cells, using CTCF antibody (TA375185) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 5s.

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  Confocal immunofluorescence analysis of Hela cells using CTCF Polyclonal Antibody (TA375185) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Confocal immunofluorescence analysis of U-2 OS cells using CTCF Polyclonal Antibody (TA375185) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody (TA375185). Western blot was performed from the immunoprecipitate using CTCF antibody (TA375185) at a dilition of 1:1000.

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  Chromatin immunoprecipitation analysis of extracts of 293T cells, using CTCF antibody (TA375185) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Specifications

Product Data

• Applications: ChIP, ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:1000 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:100
  ChIP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 45kDa/82kDa
• Gene Name: CCCTC-binding factor
• Database Link:
  Entrez Gene 10664 Human
  UniProt ID P49711
• Background: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Synonyms: CTCF