CCL3 Rabbit Polyclonal Antibody

CAT#: TA374358

CCL3 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of THP-1 cells, using CCL3 antibody (TA374358) at 1:1000 dilution.THP-1 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

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  Immunofluorescence analysis of C6 cells using CCL3 Rabbit pAb (TA374358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH-3T3 cells using CCL3 Rabbit pAb (TA374358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using CCL3 Rabbit pAb (TA374358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-92 of human CCL3 (NP_002974.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 10kDa
• Gene Name: C-C motif chemokine ligand 3
• Database Link:
  Entrez Gene 6348 Human
  UniProt ID P10147
• Background: This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.
• Synonyms: G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3; SIS-beta