HLA-DRB1 Rabbit Polyclonal Antibody

CAT#: TA365996

HLA-DRB1 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: Human spleen tissue and A375 cell lysates Primary antibody: TA365996 (HLA-DRB1 Antibody) at dilution 1/500 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA365996 (HLA-DRB1 Antibody) at dilution 1/65 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA365996 (HLA-DRB1 Antibody) at dilution 1/65, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA365996 (HLA-DRB1 Antibody) at dilution 1/65 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA365996 (HLA-DRB1 Antibody) at dilution 1/65, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Human spleen tissue and A375 cell lysates
  IHC: 50-300
  Positive control: Human tonsil
  Predicted cell location: Cytoplasm and Cell membrane
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human HLA-DRB1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 30 kDa
• Gene Name: major histocompatibility complex, class II, DR beta 1
• Database Link:
  Entrez Gene 3123 Human
  UniProt ID Q5Y7A7
• Background: HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
• Synonyms: HLA-DR1B