Tubulin Polymerization Promoting Protein (TPPP) Rabbit Polyclonal Antibody

CAT#: TA365281

TPPP rabbit polyclonal antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-2: Mouse brain tissue and Rat brain tissue Primary antibody: TA365281 (TPPP Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 seconds

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA365281 (TPPP Antibody) at dilution 1/25 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA365281 (TPPP Antibody) at dilution 1/25, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA365281 (TPPP Antibody) at dilution 1/25 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA365281 (TPPP Antibody) at dilution 1/25, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Mouse brain tissue and Rat brain tissue
  IHC: 25-100
  Positive control: Human lung cancer
  Predicted cell location: Cytoplasm or Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length fusion protein
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 24 kDa
• Gene Name: tubulin polymerization promoting protein
• Database Link:
  Entrez Gene 11076 Human
  UniProt ID O94811
• Background: Tubulin family members are globular proteins important in the assembly of microtubules. Microtubules are structural components that play important roles in mitosis, cytokinesis and vesicle transport. TPPP (Tubulin polymerization-promoting protein), also known as p24 and p25, is a widely expressed 219 amino acid protein found in the perinuclear region of the cytoplasm. TPPP may form dimers and functions in polymerizing tubulin into double-walled tubules, polymorphic aggregates, or stabilized blocks. TPPP overexpression prevents formation of the mitotic spindle assembly and breakdown of the nuclear envelope. TPPP is phosphorylated by TPK II and is encoded by a gene that maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
• Synonyms: p24; p25; p25-alpha; p25alpha; TPPP/p25; TPPP1