Adipose Triglyceride Lipase (PNPLA2) Mouse Monoclonal Antibody [Clone ID: AT18E6]

CAT#: TA355162

Adipose Triglyceride Lipase (PNPLA2) mouse monoclonal antibody, clone AT18E6

Product Images

The A431 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with anti-human ATGL antibody at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

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  Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with anti-human ATGL antibody at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Specifications

Product Data

• Clone Name: AT18E6
• Applications: ELISA, FC, ICC, WB
• Recommended Dilution: Western blot: 1/1000-1/2000
• Reactivities: Human, Mouse
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant human PNPLA2 (30-504aa) purified from E. coli
• Specificity: The antibody recognizes PNPLA2 (ATGL).
• Formulation: Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 55 kDa
• Gene Name: patatin like phospholipase domain containing 2
• Database Link:
  NP_065109
  Entrez Gene 57104 Human
  UniProt ID Q96AD5
• Background: Adipose triglyceride lipase (ATGL) is a 504 amino acid protein that is highly expressed in mouse and human adipose tissue. ATGL is catalyzes the initial step in triglyceride hydrolysis in adipocyte lipid droplets and has acylglycerol transacylase activity. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and hormone-sensitive lipase coordinately catabolize stored triglycerides in adipose tissue of mammals.
• Synonyms: 1110001C14Rik; ATGL; DESNUTRIN; DKFZp667M109; FP17548; IPLA2-zeta; PEDF-R; TTS-2.2; TTS2; TTS2.2