TIM 3 (HAVCR2) Rabbit Polyclonal Antibody

CAT#: TA355031

Rabbit Polyclonal Anti-TIM3 Antibody

Product Images

Western blot analysis of TIM3 in Jurkat cell lysate with TIM3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of TIM3 in human spleen tissue with TIM3 antibody at 2 ug/ml.

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  Immunofluorescence of TIM3 in human spleen tissue with TIM3 antibody at 20 ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1-2µg/mL. IHC: 2µg/mL. Immunoflorescence start at 20µg/mL.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: TIM3 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM3. The immunogen is located within amino acids 60 - 110 of TIM3.
• Formulation: TIM3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: TIM3 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 33 kDa; Observed: 37 kDa
• Gene Name: hepatitis A virus cellular receptor 2
• Database Link:
  NP_116171
  Entrez Gene 84868 Human
  UniProt ID Q8TDQ0
• Background: The immune checkpoint protein TIM3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-? producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).
• Synonyms: FLJ14428; HAVcr-2; KIM-3; Tim-3; TIM3; TIMD-3; TIMD3
• Note: TIM3 antibody can be used for Western blot at 1 - 2 µg/mL. Antibody can also be used for Immunohistochemistry at 2 µg/mL. For Immunoflorescence start at 20 µg/mL.