HDJ2 (DNAJA1) Rabbit Polyclonal Antibody

CAT#: TA350069

Rabbit Polyclonal Anti-DNAJA1 Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-4: HepG2 cells Raji cells A431 cells 231 cells Primary antibody: TA350069 (DNAJA1 Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA350069 (DNAJA1 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA350069 (DNAJA1 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA350069 (DNAJA1 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA350069 (DNAJA1 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: HepG2, Raji, A431 and 231 cells
  IHC: 100-300
  Positive control: Human colon cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human DNAJA1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: DnaJ heat shock protein family (Hsp40) member A1
• Database Link:
  NP_001530
  Entrez Gene 3301 Human
  UniProt ID P31689
• Background: DnaJ-like proteins interact with HSP 70 molecular chaperones and function to facilitate protein folding and mitochondrial protein import. HSP 40-4, also known as HDJ2, is the human DnaJ homolog that functions as a co-chaperone with a cysteine-rich zinc finger domain. The cellular redox enzyme thioredoxin interacts with HSP 40-4, and oxidation and reduction reversibly regulate HSP 40-4 function in response to the changing redox states of the cell. The zinc finger domain of HSP 40-4 may act as a redox sensor of chaperone-mediated protein-folding machinery, since HSP 40-4 inactivation leads to the oxidation of cysteine thiols and a simultaneous release of coordinated zinc. Loss of the HSP 40-4 protein may be linked to severe defects in spermatogenesis that involve aberrant androgen signaling.
• Synonyms: DJ-2; DjA1; hDJ-2; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7

Reference Data

• Protein Families: Druggable Genome