MIRO1 (RHOT1) Rabbit Polyclonal Antibody

CAT#: TA349118

Rabbit Polyclonal Anti-RHOT1 Antibody

Product Images

Western blot analysis of RHOT1 in rat brain tissue lysate with RHOT1 antibody at 1 ug/mL.

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  Immunohistochemistry of RHOT in mouse brain tissue with RHOT antibody at 5 ug/mL.

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  Immunofluorescence of RHOT in mouse brain tissue with RHOT antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: RHOT1 antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOT1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: RHOT1 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 76 kDa; Observed: 78 kDa
• Gene Name: ras homolog family member T1
• Database Link:
  NP_001028740
  Entrez Gene 55288 Human
  UniProt ID Q8IXI2
• Background: The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1). RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3). Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4).
• Synonyms: ARHT1; MIRO-1; MIRO1