SHARP1 (BHLHE41) Rabbit Polyclonal Antibody

SKU TA336739

Rabbit Polyclonal DEC2/SHARP1 Antibody

Product Data

• Application: IHC, Simple Western, WB
• Recommended Dilution: Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:50, Immunohistochemistry: 1:100, Western Blot: 1 - 2 ug/ml
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal portion of the human PAI1/Serpine 1 protein (between residues 1-75) [UniProt Q9C0J9]
• Buffer: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 50 kDa
• Gene Name: basic helix-loop-helix family member e41
• Database Link:
  NP_110389
  Q9C0J9
• Background: SHARP1 also known as DEC2 and BHLHe41 encodes a transcription factor that belongs to the Hairy/Enhancer of Split subfamily of basic helix-loop-helix factors (bHLH). However, sequence alignment shows that SHARP-1 is only distantly related to these proteins, exhibiting 37-42% sequence identity within the bHLH domain. Unlike most bHLH proteins, SHARP-1 is not expressed in neuronal progenitor cells or early differentiating neurons but is restricted to a subset of neurons of the postnatal central nervous system. Recent studies have shown DEC2 plays a regulatory role in clockwork system along with Clock and Bmal1. DEC1, a structurally related protein also known as SHARP2 along with DEC2 works as the negative regulated of molecular clock. Mutations in this gene (hDEC2-P385R) are associated with short sleep phenotypes in human and transgenic mice carrying this mutation show increased vigilance time and less sleep time than control mice.
• Synonyms: BHLHB3; DEC2; hDEC2; SHARP1

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Circadian rhythm - mammal