HIF-1 alpha (HIF1A) Rabbit Polyclonal Antibody

CAT#: TA336308

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Rabbit Polyclonal HIF-1 alpha Antibody



USD 479.00


Availability*
2 Weeks

Size
    • 100 ul


Specifications

Product Data
Applications IF, IHC, WB
Recommended Dilution WB: 1:500-1:2000, IF: 1:100, IHC: 5-10 ug/ml, IHC-F: 5-10 ug/ml, IHC-P: 5-10 ug/ml
Reactivity Goat, Hamster, Human, Mouse, Primate, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Immunogen A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein. [UniProt# Q61221]
Formulation Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 115 kDa
Gene Name hypoxia inducible factor 1 alpha subunit
Background HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
Synonyms bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Note This HIF-1 alpha antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded and frozen sections and Western blot, recognizing a band in hypoxic samples at ~115 kDa. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples. HIF-1 alpha Protein NBC1-18422 has been successfully used for antibody preadsorption/blocking assay in IHC-P with Hif-1alpha antibody NB100-479 (refer to product review by Yves Heremans on the datasheet of NBC1-18422).
Reference Data
Protein Families Transcription Factors
Protein Pathways mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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