IRE1 (ERN1) Rabbit Polyclonal Antibody

SKU TA336286

Rabbit Polyclonal IRE1 alpha [p Ser724] Antibody

Product Data

• Application: ChIP, ELISA, ICC/IF, IHC, Immunoblotting, IP, WB
• Recommended Dilution: Western Blot: 1:500 - 1:1000, Knockdown Validated, Immunocytochemistry/ Immunofluorescence: 1:10 - 1:500, Chromatin Immunoprecipitation (ChIP), Immunoblotting, In vitro assay, Immunohistochemistry-Paraffin: 1:10 - 1:500, Immunohistochemistry-Frozen: 1:10 - 1:500, ELISA: 1:100 - 1:2000, Immunohistochemistry: 1:10 - 1:500, Immunoprecipitation: 1:10 - 1:500
• Reactivity: Human, Mouse, Rabbit (Does not react with: Primate), Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide surrounding the phosphorylated serine 724 of the human IRE1 alpha protein. [Swiss-Prot #O75460]
• Buffer: PBS, 0.01% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: endoplasmic reticulum to nucleus signaling 1
• Database Link:
  NP_001424
  O75460
• Background: IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. It is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities. mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER.
• Synonyms: hIRE1p; IRE1; IRE1a; IRE1P
• Note: This IRE1 pS724 antibody is useful for Western blot, ELISA, and Immunohistochemistry paraffin embedded sections.(PMID: 19264902) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24823368)

Reference Data

• Protein Families: Protein Kinase, Transmembrane
• Protein Pathways: Alzheimer's disease