B3galt5 Rat Monoclonal Antibody [Clone ID: MC-631]

CAT#: TA336273

Rat Monoclonal SSEA-3 Antibody (MC-631)

Product Images

Flow Cytometry: SSEA-3 Antibody (MC-631) TA336273 - A surface stain was performed on Jurkat cells with SSEA-3 Antibody (MC-631) TA336273AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

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  Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) TA336273 - Analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody.

Specifications

Product Data

• Clone Name: MC-631
• Applications: CyTOF-ready, FC, ICC/IF, IHC, IP, WB
• Recommended Dilution: Western Blot: 1:500, Immunohistochemistry-Paraffin: 1:10-1:500, CyTOF-ready, Immunohistochemistry-Frozen: 1:10-1:500, Flow Cytometry: 10-20 ug/ml, Immunohistochemistry: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 1:50-1:100, Immunoprecipitation: 1:10-1:500
• Reactivities: Human, Mouse
• Host: Rat
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: 4-8 cell stage mouse embryos.
• Formulation: Tris-glycine, 150mM NaCl pH 7.5, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: IgM purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5
• Database Link:
  NP_001116465
  Entrez Gene 10317 HumanEntrez Gene 93961 Mouse
  UniProt ID Q9JI67
• Background: SSEA-3 (stage-specific embryonic antigen-3) is a member of SSEAs which are present on eggs as well as preimplantation embryos at specific stages of early development. SSEAs are glycan epitopes which resides on glycoproteins/glycolipids of mammalian cells, and the epitope of several of these antigens consists solely of sugar residues. SSEA-3 antigen was identified following immunization of rats with 4-8 cell mouse embryos and was present on glycolipids of human teratocarcinoma cells. During protein glycosylation, beta-1,3-galactosyltransferase 5 catalyzes the transfer of Gal to the terminal GalNAc unit of the globoside GB4, thereby synthesizing glycolipid GB5 which is SSEA-3. In its functions. SSEA-3 has been proposed to involve in the process of cell differentiation. SSEA3 expression is mostly restricted to the cytoplasm or apical surface of epithelial cells and it has been reported to express in various normal tissues with high expression levels in kidney. SSEA3 has been reported to be expressed in the ascites fluids from patients of hepatoma or pancreatic cancer, breast cancer including breast cancer stem cells.
• Synonyms: b3Gal-T5; B3GalT-V; B3GalTx; B3T5; Beta-3-Gx-T5; beta3Gal-T5; GLCT5; OTTHUMP00000109186
• Note: This SSEA3 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin sections, Immunoprecipitation and Western Blot.