Cripto1 (TDGF1) Rabbit Polyclonal Antibody

CAT#: TA336263

Rabbit Polyclonal CRIPTO Antibody

Product Images

Flow Cytometry: CRIPTO Antibody TA336263 - An intracellular stain was performed on Jurkat with TA336263 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

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  Flow Cytometry: CRIPTO Antibody TA336263 - An intracellular stain was performed on HeLa cells with TA336263PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin

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  Western Blot: CRIPTO Antibody TA336263 - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

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  Immunocytochemistry/Immunofluorescence: CRIPTO Antibody TA336263 - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Specifications

Product Data

• Applications: FC, ICC/IF, WB
• Recommended Dilution: Flow Cytometry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:1000, Western Blot: 2 ug/ml
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an N-terminal portion of mouse Cripto1 (between residues 1-50). [UniProt# P51865]
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18 kDa
• Gene Name: teratocarcinoma-derived growth factor 1
• Database Link:
  NP_003203
  Entrez Gene 21667 MouseEntrez Gene 6997 Human
  UniProt ID P13385
• Background: Cripto-1 (teratocarcinoma-derived growth factor-1) is the original member of EGF-cripto-1-FRL-1-cryptic (CFC) family of vertebrate signaling molecules which is essential for embryonic development and it implicates in carcinogenesis also. Cripto1 functions as a co-receptor for NODAL (TGF-beta member) and is essential for mesoderm as well as endoderm formation and anterior-posterior and left-right axis establishment. After executing key functions during embryonic development, it dramatically disappears in adult tissues (except stem cells). Cripto-1 modulate signaling of TGF-beta family members (NODAL, GDF-1/-3, Activin, TGF-beta1 etc), and activate c-src/MAPK/AKT pathway in a Glypican-1/GRP78-dependent manner, and cross-talks with erbB4, Wnt/beta-catenin, NOTCH, Caveolin-1, and Apelin/putative receptor protein related to angiotensin-type I receptor pathways. During embryonic development, Cripto-1 together with Nodal controls cell migration and EMT, whereas, postnatally, it regulates the branching morphogenesis of mammary gland. Moreover, in adult life it gets re -expressed in several type of tumors wherein it promotes cell proliferation, migration, invasion, EMT, and angiogenesis.
• Synonyms: CR; CRGF; CRIPTO
• Note: This Cripto1 antibody is useful in Immunocytochemistry/Immunofluorescence, Flow Cytometry and Western Blot. In Western blot a band is seen at ~18 kDa representing the core form of Cripto1, and at ~36 kDa reporesenting the glycosylated/ post-translationally modified form.

Reference Data

• Protein Families: Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane