GABA A Receptor alpha 3 (GABRA3) Rabbit Polyclonal Antibody

CAT#: TA328699

Rabbit Polyclonal Anti-GABA (A) alpha3 Receptor (extracellular)

Product Images

Western blot analysis of rat brain membranes: 1. Anti-GABA(A) a3 Receptor (extracellular) antibody, (1:200). 2. Anti-GABA(A) a3 Receptor (extracellular) antibody preincubated with the control peptide antigen.

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  Expression of GABA(A) a3 Receptor in rat cerebellum. Immunohistochemical staining of rat cerebellum using Anti-GABA(A) a3 Receptor (extracellular), (green), (1:100). GABA(A) a3 Receptor is localized to the molecular layer (asterisk) and a portion of the Purkinje cell body (arrows), which is outlined by axonal staining using mouse anti-neurofilament 200 (red).

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  Expression of GABA(A) a3 Receptor in rat insulinoma cell line. Immunocytochemical staining of intact living rat RIN-m cells using Anti-GABA(A) a3 Receptor (extracellular) antibody, (1:50) followed by goat anti-rabbit-AlexaFluor-550 secondary antibody. Extracellular staining (red) merged with live view of the cells.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide QGESRRQEPGDFVKQ(C), corresponding to amino acid residues 29-43 of human GABA (A) a3 Receptor. Extracellular, N-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Reconstitution Method: Add 50 ul double distilled water (DDW) to the lyophilized powder.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: gamma-aminobutyric acid type A receptor alpha3 subunit
• Database Link:
  NP_000799
  Entrez Gene 14396 MouseEntrez Gene 24947 RatEntrez Gene 2556 Human
  UniProt ID P34903
• Background: The neurotransmitter GABA (?-aminobutiric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl channel, producing an increase in membrane conductance that results in inhibition of neural activity. There are two major types of GABA receptors: the ionotropic GABAA receptors (GABAAR) and the metabotropic GABAB receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily. GABAARs are heteropentamers, in which all five subunits contribute to formation of the pore. Eight subunit isoforms have been cloned: a, Ã?, ?, d, e, p, ?, and ?.Six a subunits isoforms (a1-a6) have been shown to exist in mammals. In most cases, the native GABAA receptors consist of 2a, 2Ã?, and 1? subunits. The a3-subunit is highly expressed during development (along with a2 and a5) and then declines in adulthood, where the a1-subunit becomes predominant. The failure to complete this switch could be a major predispositional factor in the development of temporal lobe epilepsy.
• Synonyms: MGC33793
• Note: This antibody was tested in live cell imaging. Please see IF/ICC data for detail.

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction