Transglutaminase 2 (TGM2) Rabbit Polyclonal Antibody

CAT#: TA324993

Rabbit polyclonal Transglutaminase (TGM2) Antibody (Center K444)

Product Images

TGM2 Antibody (Center K444) (Cat. #TA324993) western blot analysis in HUVEC, K562 cell line lysates (35ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow).

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  Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of U251 cell stained with TGM2 Antibody (Center K444) (Cat#TA324993).U251 cells were incubated with TGM2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).TGM2 immunoreactivity is localized to Cytoplasm significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, IHC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This Transglutaminase (TGM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-458 amino acids from the Central region of human Transglutaminase (TGM2).
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77329 Da
• Gene Name: transglutaminase 2
• Database Link:
  NP_004604
  Entrez Gene 7052 Human
  UniProt ID P21980
• Synonyms: G-ALPHA-h; GNAH; HEL-S-45; TG2; TGC

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease