PKC nu (PRKD3) Rabbit Polyclonal Antibody

CAT#: TA324894

Rabbit polyclonal PKC nu Antibody

Product Images

Western blot analysis of anti-PKCnu Pab (Cat. #TA324894) in lysate of HL60 cells stimulated with PMA (lane A) and mouse brain tissue lysate (lane B). PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

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  Upper panel, WB of GFP fusion protein expression in Panc-1 cells by using anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: GFP-PKD-transfected cells; lanes 3 and 7: GFP-PKD2-transfected cells; lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, WB of GFP fusion protein expression in Panc-1 cells by using PKD3 N-term (TA324894) and C-term (AP7025b) antibodies. Lower panel, IF of GFP-PKD3 protein expression in Panc-1 cells by using TA324894 and AP7025b antibodies.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This PKC-nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-384 amino acids from human PKC-nu.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 100471 Da
• Gene Name: protein kinase D3
• Database Link:
  NP_005804
  Entrez Gene 23683 Human
  UniProt ID O94806
• Synonyms: EPK2; nPKC-NU; PKC-NU; PKD3; PRKCN

Reference Data

• Protein Families: Druggable Genome, Protein Kinase