GRK4 Rabbit Polyclonal Antibody

CAT#: TA323501

Anti-GRK4 Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: PC3 cell lysate Primary antibody: TA323501 (GRK4 Antibody) at dilution 1/700 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 2 minutes

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  Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TA323501 (GRK4 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TA323501 (GRK4 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: PC3 cell lysate
  IHC: 50-100
  Positive control: Human colorectal cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 36-145 amino acids of human G protein-coupled receptor kinase 4
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66 kDa
• Gene Name: G protein-coupled receptor kinase 4
• Database Link:
  NP_001004056
  Entrez Gene 2868 Human
  UniProt ID P32298
• Background: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.
• Synonyms: GPRK2L; GPRK4; GRK4a; IT11

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis