MCM4 Rabbit Polyclonal Antibody

CAT#: TA321984

Anti-MCM4 Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 50 μg Lane 1-3: NIH/3T3 cells Hela cells K562 cells Primary antibody: TA321984 (MCM4 Antibody) at dilution 1/700 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA321984 (MCM4 Antibody) at dilution 1/40 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA321984 (MCM4 Antibody) at dilution 1/40, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA321984 (MCM4 Antibody) at dilution 1/40 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA321984 (MCM4 Antibody) at dilution 1/40, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: NIH/3T3, Hela and K562 cells
  IHC: 50-200
  Positive control: Human brain
  Predicted cell location: Cytoplasm, Nucleus
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 837-850 amino acids of Human minichromosome maintenance complex component 4
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 97 kDa
• Gene Name: minichromosome maintenance complex component 4
• Database Link:
  NP_877423
  Entrez Gene 17217 MouseEntrez Gene 4173 Human
  UniProt ID P33991
• Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2; 6 and 7 proteins possesses DNA helicase activity; and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK; a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
• Synonyms: CDC21; CDC54; hCdc21; NKCD; NKGCD; P1-CDC21

Reference Data

• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication