ZBTB4 Rabbit Polyclonal Antibody

CAT#: TA319989

Rabbit Polyclonal ZBTB4 Antibody

Product Images

Western blot analysis of ZBTB4 in SK-N-SH cell lysate with ZBTB4 antibody at 1 ug/mL.

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  Immunohistochemistry of ZBTB4 in human brain tissue with ZBTB4 antibody at 2.5 ug/mL.

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  Immunofluorescence of ZBTB4 in human brain tissue with ZBTB4 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ZBTB4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human ZBTB4.
• Formulation: ZBTB4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: ZBTB4 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: zinc finger and BTB domain containing 4
• Database Link:
  NP_065950
  Entrez Gene 75580 MouseEntrez Gene 57659 Human
  UniProt ID Q9P1Z0
• Background: ZBTB4 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions; this degradation increases with DNA damage.
• Synonyms: KAISO-L1; ZNF903