NCBP1 Rabbit Polyclonal Antibody

CAT#: TA319688

Rabbit Polyclonal NCBP1 Antibody

Product Images

Western blot analysis of NCBP1 in HeLa cell lysate with NCBP1 antibody at (A) 1 and (B) 2 ug/mL.

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  Immunocytochemistry of NCBP1 in HeLa cells with NCBP1 antibody at 2.5 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 - 2 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: NCBP1 antibody was raised against a 17 amino acid peptide near the center of human NCBP1.
• Formulation: NCBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: NCBP1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 87 kDa; Observed: 87 kDa
• Gene Name: nuclear cap binding protein subunit 1
• Database Link:
  NP_002477
  Entrez Gene 298075 RatEntrez Gene 433702 MouseEntrez Gene 4686 Human
  UniProt ID Q09161
• Background: NCBP1 Antibody: NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1,2). Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3).
• Synonyms: CBP80; NCBP; Sto1

Reference Data

• Protein Pathways: Spliceosome