Osteopontin (SPP1) Mouse Monoclonal Antibody [Clone ID: 1B20]

CAT#: TA309877

Mouse Monoclonal Osteopontin Antibody (1B20)

Product Images

Immunocytochemistry/Immunofluorescence: Osteopontin/OPN Antibody (1B20) - BSA Free TA309877 - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

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  Simple Western: Osteopontin/OPN Antibody (1B20) - BSA Free TA309877 - Simple Western lane view shows a specific band for Osteopontin in 0.5 mg/ml of Human Breast Cancer lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

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  Western Blot: Osteopontin/OPN Antibody (1B20) - BSA Free TA309877 - Analysis of Osteopontin expression in U2OS whole cell lysate.

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  Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free TA309877 - Analysis of Osteopontin on human lung adenocarcinoma.

Specifications

Product Data

• Clone Name: 1B20
• Applications: ICC/IF, IHC, IP, Simple Western, WB
• Recommended Dilution: Immunohistochemistry-Paraffin: 1:100, Immunohistochemistry-Frozen, Simple Western: 1:20, Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:50, Immunoprecipitation: 1:10-1:500, Western Blot: 1:1000
• Reactivities: Human, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].
• Formulation: Tris-glycine, 150mM NaCl and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: secreted phosphoprotein 1
• Database Link:
  NP_000573
  Entrez Gene 25353 RatEntrez Gene 6696 Human
  UniProt ID P10451
• Synonyms: BNSP; BSPI; ETA-1; OPN

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway