Lysosomal acid lipase (LIPA) Rabbit Polyclonal Antibody

CAT#: TA309730

Rabbit Polyclonal Lipase A Antibody

Product Images

Simple Western: Lipase A Antibody TA309730 - Image shows a specific band for Lipase A in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunocytochemistry/Immunofluorescence: Lipase A Antibody TA309730 - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lipase A Antibody TA309730 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

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  Immunohistochemistry: Lipase A Antibody TA309730 - Staining of LIPA in mouse stomach smooth muscle.

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  Immunohistochemistry-Paraffin: Lipase A Antibody - BSA Free TA309730 - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of Lipase A antibody (TA309730). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

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  Western Blot: Lipase A Antibody TA309730 - Detection of LIPA in HepG2 whole cell extract.

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Specifications

Product Data

• Applications: ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Immunohistochemistry: 1:100, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:10000, Western Blot: 1 - 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:100 - 1:500
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A genomic peptide made to an internal region of the human Lysosomal acid lipase protein (within residues 150-300). [Swiss-Prot P38571]
• Formulation: PBS, 0.02% Sodium Azide
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lipase A, lysosomal acid type
• Database Link:
  NP_000226
  Entrez Gene 16889 MouseEntrez Gene 3988 Human
  UniProt ID P38571
• Synonyms: CESD; LAL

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysosome, Steroid biosynthesis