Non Neuronal Enolase (ENO1) Rabbit Polyclonal Antibody

CAT#: TA308873

Rabbit Polyclonal antibody to ENO1 (enolase 1, (alpha))

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308873 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: A431. B: H1299. 10% SDS PAGE. TA308873 diluted at 1:3000

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  Immunohistochemical analysis of paraffin-embedded gastric tissue, using ENO1 (TA308873) antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using enolase 1 (TA308873) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Dot:blot:; ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse (Predicted: Chimpanzee, Rhesus Monkey)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 199 and 434 of ENO1 (Uniprot ID#P06733)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47 kDa
• Gene Name: enolase 1
• Database Link:
  NP_001419
  Entrez Gene 13806 MouseEntrez Gene 2023 Human
  UniProt ID P06733
• Background: This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]
• Synonyms: ENO1L1; MPB1; NNE; PPH
• Note: Seq homology of immunogen across species: Rhesus Monkey (100%), Chimpanzee (100%)

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation