RPB2 (POLR2B) Rabbit Polyclonal Antibody

CAT#: TA308532

Rabbit Polyclonal antibody to POLR2B (polymerase (RNA) II (DNA directed) polypeptide B, 140kDa)

Product Images

Sample (30 ug of whole cell lysate). A: BCL-1. 5% SDS PAGE. TA308532 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: H1299. 5% SDS PAGE. TA308532 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using POLR2B (TA308532) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using POLR2B (TA308532) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: Rat, Bovine, Rhesus Monkey)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 172 of RNA polymerase IIB (Uniprot ID#P30876)
• Formulation: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 134 kDa
• Gene Name: polymerase (RNA) II subunit B
• Database Link:
  NP_000929
  Entrez Gene 289561 RatEntrez Gene 5431 Human
  UniProt ID P30876
• Background: This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq]
• Synonyms: hRPB140; POL2RB; RPB2
• Note: Seq homology of immunogen across species: Rat (100%), Rhesus Monkey (100%), Bovine (100%)

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase