RFC4 Rabbit Polyclonal Antibody

CAT#: TA308528

Rabbit Polyclonal antibody to RFC4 (replication factor C (activator 1) 4, 37kDa)

Product Images

Sample (30 ug of whole cell lysate). A: H1299. B: Hela. C: Hep G2. 10% SDS PAGE. TA308528 diluted at 1:1000.

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  Sample (50 ug of whole cell lysate). A: mouse liver. 10% SDS PAGE. TA308528 diluted at 1:500.

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  Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using RFC4 (TA308528) antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed A549, using RFC4 (TA308528) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse (Predicted: Rat, Xenopus, X. tropicalis)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 18 and 323 of RFC4 (Uniprot ID#P35249)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40 kDa
• Gene Name: replication factor C subunit 4
• Database Link:
  NP_002907
  Entrez Gene 106344 MouseEntrez Gene 288003 RatEntrez Gene 5984 Human
  UniProt ID P35249
• Background: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]
• Synonyms: A1; RFC37
• Note: Seq homology of immunogen across species: Rat (92%), Xenopus laevis (84%), Xenopus tropicalis (87%)

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair