Flightless I (FLII) Rabbit Polyclonal Antibody

CAT#: TA308275

Rabbit Polyclonal antibody to Flightless I (flightless I homolog (Drosophila))

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. TA308275 diluted at 1:1000.

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  Sample (30 ug whole cell lysate). A:Raji. 5% SDS PAGE. TA308275 diluted at 1:1000

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  Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I (TA308275) antibody at 1:200 dilution.

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  Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless I (TA308275) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse (Predicted: Chicken, Rat, X. tropicalis)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 435 and 778 of Flightless I (Uniprot ID#Q13045)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 145 kDa
• Gene Name: FLII, actin remodeling protein
• Database Link:
  NP_002009
  Entrez Gene 14248 MouseEntrez Gene 287375 RatEntrez Gene 2314 Human
  UniProt ID Q13045
• Background: This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq]
• Synonyms: FLI; Fli1; FLIL
• Note: Seq homology of immunogen across species: Chicken (87%), Rat (96%), Xenopus tropicalis (81%)

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors