PGD Rabbit Polyclonal Antibody

CAT#: TA308104

Rabbit Polyclonal antibody to PGD (phosphogluconate dehydrogenase)

Product Images

Sample (30 ug whole cell lysate). A:293T. B:MOLT4. C:Raji. 10% SDS PAGE. TA308104 diluted at 1:1000

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  PGD antibody [N1N3] detects PGD protein at cytoplasm on A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded A549 xenograft. PGD antibody [N1N3] (TA308104) dilution: 1:500.

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  PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PGD protein stained by PGD antibody [N1N3] (TA308104) diluted at 1:500. Blue: Hoechst 33342 staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: Mouse, Chicken, Xenopus, Zebrafish, Bovine)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 201 of PGD (Uniprot ID#P52209)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53 kDa
• Gene Name: phosphogluconate dehydrogenase
• Database Link:
  NP_002622
  Entrez Gene 110208 MouseEntrez Gene 5226 Human
  UniProt ID P52209
• Background: 6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. [provided by RefSeq]
• Synonyms: 6PGD
• Note: Seq homology of immunogen across species: Mouse (97%), Chicken (90%), Xenopus laevis (85%), Zebrafish (86%), Bovine (94%)

Reference Data

• Protein Pathways: Glutathione metabolism, Metabolic pathways, Pentose phosphate pathway