POLR3A Rabbit Polyclonal Antibody

CAT#: TA308085

Rabbit polyclonal antibody to POLR3A (polymerase (RNA) III (DNA directed) polypeptide A, 155kDa)

Product Images

Sample (30 ug of whole cell lysate). A: HCT116. 5% SDS PAGE. TA308085 diluted at 1:500.

• 

  Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using POLR3A (TA308085) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: Mouse, Chicken, Xenopus, Bovine, X. tropicalis)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 182 and 482 of POLR3A (Uniprot ID#O14802)
• Formulation: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 156 kDa
• Gene Name: polymerase (RNA) III subunit A
• Database Link:
  NP_008986
  Entrez Gene 218832 MouseEntrez Gene 11128 Human
  UniProt ID O14802
• Synonyms: ADDH; HLD7; hRPC155; RPC1; RPC155
• Note: Seq homology of immunogen across species: Mouse (97%), Chicken (97%), Xenopus laevis (94%), Bovine (98%), Xenopus tropicalis (94%)

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase