BRSK1 Rabbit Polyclonal Antibody

CAT#: TA306410

Rabbit Polyclonal BRSK1 Antibody

Product Images

Western blot analysis of BRSK1 in human brain tissue lysate with BRSK1 antibody at (A) 0.5 and (B) 1 ug/ml.

• 

  Immunohistochemistry of BRSK1 in human brain tissue with BRSK1 antibody at 2.5 ug/ml.

• 

  Immunofluorescence of BRSK1 in Human Brain cells with BRSK1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: BRSK1 antibody was raised against a 28 amino acid peptide from near the carboxy terminus of human BRSK1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BR serine/threonine kinase 1
• Database Link:
  NP_115806
  Entrez Gene 381979 MouseEntrez Gene 499073 RatEntrez Gene 84446 Human
  UniProt ID Q8TDC3
• Background: BRSK1 was initially identified as a mammalian homolog to the fission yeast S. pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function. More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.
• Synonyms: hSAD1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase