MDA5 (IFIH1) Rabbit Polyclonal Antibody

SKU TA306393

Rabbit Polyclonal MDA5 Antibody

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: WB: 1 - 4 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: MDA5 antibody was raised against a 16 amino acid peptide from near the center of human MDA5.
• Buffer: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: interferon induced with helicase C domain 1
• Database Link:
  NP_071451
  Q9BYX4
• Background: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.
• Synonyms: AGS7; Hlcd; IDDM19; MDA-5; MDA5; RLR-2

Reference Data

• Protein Pathways: RIG-I-like receptor signaling pathway