TRIM5 alpha (TRIM5) Rabbit Polyclonal Antibody

CAT#: TA306151

Rabbit Polyclonal TRIM5 delta Antibody

Product Images

Western blot analysis of TRIM5 delta expression in mouse brain cell lysate with TRIM5 delta antibody at 2 ug/ml.

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  Immunohistochemistry of TRIM5d in mouse brain tissue with TRIM5d antibody at 1 ug/ml.

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  Immunofluorescence of TRIM5 delta in mouse brain tissue with TRIM5 delta antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 2 ug/mL, ICC: 1 ug/mL, IF: 10 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: TRIM5 delta antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy-terminus of human TRIM5 delta.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tripartite motif containing 5
• Database Link:
  NP_149084
  Entrez Gene 85363 Human
  UniProt ID Q9C035
• Background: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region (1). TRIM5 has five alternately spliced isoforms, the longest of which is the ? variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1). Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1 (2,3), albeit at differing efficiencies. The TRIM5delta isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5delta also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner (4).
• Synonyms: RNF88; TRIM5alpha