CRF1 (CRHR1) Goat Polyclonal Antibody

CAT#: TA303282

Goat Polyclonal Antibody against CRHR1

Product Images

TA303282 (0.1ug/ml) staining of human colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Confocal image (3um) of TA303282 (0.3ug/ml) stainiug inugreen a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA.

Specifications

Product Data

• Applications: IF, PEP-ELISA, WB
• Recommended Dilution: ELISA: 1:32,000, WB: 0.1-0.3µg/ml, IF: 2ug/ml.
• Reactivities: Human, Mouse (Expected from sequence similarity: Rat)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-NEEKKSKVHYH, from the internal region of the protein sequence according to NP_004373.2.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: corticotropin releasing hormone receptor 1
• Database Link:
  NP_001138618
  Entrez Gene 12921 MouseEntrez Gene 58959 RatEntrez Gene 1394 Human
  UniProt ID P34998
• Background: This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants.
• Synonyms: CRF-R; CRF-R-1; CRF-R1; CRF1; CRFR-1; CRFR1; CRH-R-1; CRH-R1; CRHR; CRHR1L
• Note: This product was successfully used on cryosection of mouse brain

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Long-term depression, Neuroactive ligand-receptor interaction