Frizzled 8 (FZD8) Goat Polyclonal Antibody

CAT#: TA303076

Goat Polyclonal Antibody against FZD8

Product Images

TA303076 (1ug/ml) staining of human pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  TA303076 (3ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:32,000. WB: 1-3µg/ml. IHC: 3µg/ml.
• Reactivities: Human (Expected from sequence similarity: Mouse, Rat, Dog, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: frizzled class receptor 8
• Database Link:
  NP_114072
  Entrez Gene 14370 MouseEntrez Gene 364754 RatEntrez Gene 487073 DogEntrez Gene 8325 Human
  UniProt ID Q9H461
• Background: This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq]
• Synonyms: FZ-8; hFZ8

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway